Spike Protein (updated pt 11)
How much and where
This post is to shed a bit of light on the mysteries of how much spike protein is being made, where it is going and how is it going there. Sadly our captured regulatory agencies did not insist the Pfizer or Moderna to do so and to date nobody has bothered doing so that I can see.
1/ I start by estimating how much spike protein is being created by the mRNA vaccines
2/We know there is 30 microgram mRNA for Pfizer
3/Molecular weight is estimated at > 1.25 x10^6 gm/mole. (~330 gm per mole of nucleotide)
This comes out to
30 x 10^-6 grams/
1.25 x 10^6 gm/mole
=24 x 10^-12 moles
6 x 10^23 molecules/mole x 24 x 10^-12 moles =14.4x 10^12 molecules mRNA (14.4trillion)
4/14.4 trillion spike protein if 1 mRNA produces 1 spike protein
A more reasonable number is 1000 spike per mRNA. But it could be anything in between
X1000 14,400 trillion spikes
X100. 1,440 trillion spikes
X10. 144 trillion spiked
We will use the bigger number. Let Pfizer prove otherwise (they know)
http://book.bionumbers.org/how-many-proteins-are-made-per-mrna-molecule/
5a/So basically 30 micrograms of mRNA will theoretically produce about 14,400 trillion individual spike proteins (if 100% efficient)
5b/how about the weight of the spike produced?
MW spike = 180 kDa
180 kDa = 3 x 10^-19 gm/molecule
14.4 x 10^15 molecules spike protein
3 x 14.4 x 10^-4 grams
43 x 10-4 grams
4,300 micrograms spike
6/Now we ask this. How many cells are transfected by the vaccines. In other words, how many mRNA molecules are in each LNP
7/Since we can estimate how many mRNA molecules there are (14.4 trillion for Pfizer), we must estimate how many LNP’s
8/First we must estimate the size of LNP
“Together with the mRNA, these components form particles of about 60–100 nm in size by using a rapid mixing production technique (Evers et al., 2018).”
9/So lets call it 80 nm and assume a spherical shape. Now we must know how much LNP is in each dose.
10/So LNP particles of 80 nm have a total weight of about 0.5 mg. Lipids are typically less dense than water but using the density of water we may estimate the volume of 0.5 mg as 500 cubic nanoliters which is 5 x 10^17 cubic nm
https://www.sciencedirect.com/science/article/pii/S0378517321003914?via%3Dihub
11/An individual 80 nm dia LNP particles volume is 256,000 cubic nm or 2.6 x 10^5 cubic nm/particle
5 x 10^17 cubic nm /2.6 x 10^5 cubic nm/particle = 2 x 10^ 12 - LNP particles
Edit 10-11-22: In one of the document dumps Pfizer spec lists LNP diameter spec as <200 nm . We really want the mean and not the upper limit but if we do the above calculationd based on 200 nm dia we get 140 billion nanoparticles (1.4 x 10^11)
We had already estimated
14,400 x 10^12 spike
14.4 x 10^12 mRNA
Reminder 10^12 is 1 trillion (big number)
12/ So basically each LNP will hold about 7 mRNA molecules (or ~ 100 if using a diameter of 200 nm) . And at most 2 x 10^12 cells will be transfected, although its likely multiple LNP will transfect a cell. We just have no way to estimate this
13/Current estimates of cells in human body are 37 x10^12, and we have 2 x 10^12 LNP and 14.4 x 10^ 12 mRNA that will produce up to 14,400 x 10^12 spike protein
14/Obviously these are ballpark numbers. Its a shame we don’t have precise numbers on these questions and so many others such where does the mRNA end up and how long before its degraded
15/ Its simply incredible to me that they were not required to do a biodistribution study on primates yo find out where the mRNA goes to after its injected.
16a/Pfizer used a luciferase mRNA but only tracked the lipids in the LNP. Moderna used a study on a different mRNA and lipids . Both of them used mice
The Moderna study tracked the H10 mRNA distribution
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5475249/#!po=0.505051
16b/here is Pfizers lipid biodistribution
Fact checkers say Pfizers study does not prove the lipid contains mRNA
Yet Pfizers Report in the March release says this
https://phmpt.org/wp-content/uploads/2022/03/125742_S1_M2_24_nonclinical-overview.pdf
17/The other thing we want to know is where does the spike protein end up? We know that once produced in the cell its presented on the cell surface (and some is chopped up and presented on MHC-I or MhC-2 molecules depending on the cell type)
18/We know that the cells transfected and which produce spike protein are destroyed by the immune cells or the cells undergo apoptosis or necrosis, and the idea is that the protein is completely destroyed
19/ yet we also have seen studies showing the S1 protein in the plasma of nurses after vaccination for up to 28 days after vaccination, and also the entire spike protein for a shorter period
https://academic.oup.com/cid/advance-article/doi/10.1093/cid/ciab465/6279075
20/ We have also seen studies on mice showing S1 can disrupt the blood brain barrier and pass into the brain, and in vitro human models
https://pubmed.ncbi.nlm.nih.gov/33053430/
https://www.nature.com/articles/s41593-020-00771-8
21/ Furthermore Dr Patterson has discovered that S1 persists in non-classical monocytes for up to 15 months after infection and is seeing the same in post-vaccination patients.
22/These monocytes repair blood vessel wall and crawl over the blood vessel and may be causing inflammation which cause long haul symptoms
23/The question for all that is how an IM injection can lead to LNP/mRNA or spike proteins into the circulatory system. Some Doctors say its not possible, they are too big but don’t mention Transcytosis
https://www.researchgate.net/publication/6671046_Transcytosis_Crossing_Cellular_Barriers
Of course, drainage from the lymph nodes is another way.
Another way not discussed is by injecting into a blood vessel. Nurses are supposed to aspirate to ensure that does not happen. But we know it can.
But oh wait, but CDC says its ok not to aspirate now.
https://www.cdc.gov/vaccines/hcp/acip-recs/general-recs/administration.html
Do they get paid by the body count?
24/How much spike protein is made outside the nasal pharynx/lungs or enters the circulation from a mild natural infection?
From this recent study we see this
At least some portion of spike antigen generated after administration of BNT162b2 becomes distributed into the blood. We detected spike antigen in 96% of vaccinees in plasma collected one to two days after the prime injection, with antigen levels reaching as high as 174 pg/mL.
25/How does that compare to what is produced
174 x10^-12 gm/cc
3 Liters plasma
174 x 10^-12 x 3,000 cc= 522 x 10^-9 gm in blood= 0.5 microgram
Above we had estimated 4,300 micrograms spike being produced, but of course this is happening over a few days or even longer period, and the spike in the plasma is being constantly reduced as its deposited, degraded or excreted
Also the estimate is a very rough number, it could be off an order of two or more in magnitude, especially if the transfection and translation rates are lower than assumed
26/. But most importantly the linked study goes on to say :
The range of spike antigen concentrations in the blood of vaccinees at this early time point largely overlaps with the range of spike antigen concentrations reported in plasma in a study of acute infection(Ogata et al., 2020),
27/. So lets look at Ogata et al., 2020)
https://academic.oup.com/clinchem/article/66/12/1562/5902449
S1 and N were detected in 41 of 64 COVID-19 positive patients (Fig. 1, B and C), who we identify as “viral-antigen positive.” Despite the presence of S1 and N in some samples, spike was only detectable in 5 of 64 COVID-19 positive patients . Spike may be undetectable in some samples since the LOD is 1 order of magnitude higher than the LOD of the S1 assay.
Hmmm. This is a far cry from full spike being detected in 96% of vaccinees,and while 2/3 had S1 full spike was undetectable in over 90% of patients
Lets look closer
....patients were grouped into 3 categories of S1 concentration: (a) 23 patients with undetectable S1concentrations (below the limit of detection), (b) 23 patients with low concentrations of S1 (6–50 pg/mL, 0.08–0.65 pmol/L), and (c) 18 patients with high concentrations of S1 (>50 pg/mL, > 0.65 pmol/L). There is a significant difference in rates of ICU admission upon presentation for the 3 patient groups based on S1 concentrations in plasma (P = 0.0107). Patients with zero, low, and high concentrations of S1 were admitted to the ICU upon presentation to the hospital at rates of 30% (7 of 23 patients), 52% (12 of 23 patients), and 77% (14 of 18 patients), respectively
So all of these had significant disease requiring hospital admission and some had no S1, while 96% of vaccinees had detectable levels of full spike at 1-2 days and 2/3 at 7 days
If I read this correctly looks to me as if those getting vaccinated are being exposed to more spike protein, at least outside the lungs and nasal-pharynx areas , than those who will get mild COVID.
Prove me wrong Pfizer. With hard data and not words
Of course, I can hear it now. “But the spike protein produced by the vaccine is safer than the spike produced by the virus. We have no data to back that up but trust the Science”.